DETECTION OF RENIN mRNA IN ALDOSTERONE‐PRODUCING ADENOMAS BY POLYMERASE CHAIN REACTION
Identifieur interne : 00D914 ( Main/Exploration ); précédent : 00D913; suivant : 00D915DETECTION OF RENIN mRNA IN ALDOSTERONE‐PRODUCING ADENOMAS BY POLYMERASE CHAIN REACTION
Auteurs : Shelley Klemm [Australie] ; Florence Pinet [France] ; Nathalie Rioual-Caroff [France] ; Terry Tunny [Australie] ; Pierre Corvol [France] ; Richard Gordon [Australie]Source :
- Clinical and Experimental Pharmacology and Physiology [ 0305-1870 ] ; 1993-05.
English descriptors
- KwdEn :
- Adenoma, Adrenal, Aldosterone, Angiotensin, Angiotensin infusion, Biochemical behaviour, Cdna, Experimental pharmacology, Hypertension, Infusion, Klemm, Mrna, Normal adrenals, Polymerase, Polymerase chain reaction, Primary aldosteronism, Primer, Renin, Renin gene expression, Renin mrna, Rnin, Tumour, Various tissues, Vivo responsiveness.
- Teeft :
- Adenoma, Adrenal, Aldosterone, Angiotensin, Angiotensin infusion, Biochemical behaviour, Cdna, Experimental pharmacology, Hypertension, Infusion, Klemm, Mrna, Normal adrenals, Polymerase, Polymerase chain reaction, Primary aldosteronism, Primer, Renin, Renin gene expression, Renin mrna, Rnin, Tumour, Various tissues, Vivo responsiveness.
Abstract
1. Ribonucleic acid (RNA) was extracted from two normal human adrenal cortices and from five aldosterone‐producing adenomas (APA). 2. The five APA could be categorized, on the basis of in vivo aldosterone responsiveness to angiotensin infusion and upright posture, into two APA responsive to both stimuli, two responsive only to angiotensin infusion, and one unresponsive to either stimulus. 3. RNA was reverse transcribed and coamplified by polymerase chain reaction (PCR) with an internal standard of renin complementary DNA (cDNA) containing a 60 base pair insertion. Renin mRNA in the APA was compared with normal adrenals. 4. Renin mRNA was greater than normal in the two APA responsive to both stimuli and less than, or similar to normal, in the two APA responsive only to angiotensin infusion. Renin mRNA was also less than, or similar to normal, in the APA unresponsive to either stimulus. 5. These findings support a possible role for adrenal renin in the development and biochemical behaviour of angiotensin‐responsive APA.
Url:
DOI: 10.1111/j.1440-1681.1993.tb01689.x
Affiliations:
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Gordon, Endocrine‐Hypertension Research Unit, Greenslopes Hospital, Greenslopes, Brisbane, Qld 4120</wicri:regionArea><wicri:noRegion>Qld 4120</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Clinical and Experimental Pharmacology and Physiology</title><title level="j" type="alt">CLINICAL EXPERIMENTAL PHARMACOLOGY PHYSIOLOGY</title><idno type="ISSN">0305-1870</idno><idno type="eISSN">1440-1681</idno><imprint><biblScope unit="vol">20</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="303">303</biblScope><biblScope unit="page" to="305">305</biblScope><biblScope unit="page-count">3</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1993-05">1993-05</date></imprint><idno type="ISSN">0305-1870</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0305-1870</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenoma</term><term>Adrenal</term><term>Aldosterone</term><term>Angiotensin</term><term>Angiotensin infusion</term><term>Biochemical behaviour</term><term>Cdna</term><term>Experimental pharmacology</term><term>Hypertension</term><term>Infusion</term><term>Klemm</term><term>Mrna</term><term>Normal adrenals</term><term>Polymerase</term><term>Polymerase chain reaction</term><term>Primary aldosteronism</term><term>Primer</term><term>Renin</term><term>Renin gene expression</term><term>Renin mrna</term><term>Rnin</term><term>Tumour</term><term>Various tissues</term><term>Vivo responsiveness</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Adenoma</term><term>Adrenal</term><term>Aldosterone</term><term>Angiotensin</term><term>Angiotensin infusion</term><term>Biochemical behaviour</term><term>Cdna</term><term>Experimental pharmacology</term><term>Hypertension</term><term>Infusion</term><term>Klemm</term><term>Mrna</term><term>Normal adrenals</term><term>Polymerase</term><term>Polymerase chain reaction</term><term>Primary aldosteronism</term><term>Primer</term><term>Renin</term><term>Renin gene expression</term><term>Renin mrna</term><term>Rnin</term><term>Tumour</term><term>Various tissues</term><term>Vivo responsiveness</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">1. Ribonucleic acid (RNA) was extracted from two normal human adrenal cortices and from five aldosterone‐producing adenomas (APA). 2. The five APA could be categorized, on the basis of in vivo aldosterone responsiveness to angiotensin infusion and upright posture, into two APA responsive to both stimuli, two responsive only to angiotensin infusion, and one unresponsive to either stimulus. 3. RNA was reverse transcribed and coamplified by polymerase chain reaction (PCR) with an internal standard of renin complementary DNA (cDNA) containing a 60 base pair insertion. Renin mRNA in the APA was compared with normal adrenals. 4. Renin mRNA was greater than normal in the two APA responsive to both stimuli and less than, or similar to normal, in the two APA responsive only to angiotensin infusion. Renin mRNA was also less than, or similar to normal, in the APA unresponsive to either stimulus. 5. These findings support a possible role for adrenal renin in the development and biochemical behaviour of angiotensin‐responsive APA.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li></settlement></list><tree><country name="Australie"><noRegion><name sortKey="Klemm, Shelley" sort="Klemm, Shelley" uniqKey="Klemm S" first="Shelley" last="Klemm">Shelley Klemm</name></noRegion><name sortKey="Gordon, Richard" sort="Gordon, Richard" uniqKey="Gordon R" first="Richard" last="Gordon">Richard Gordon</name><name sortKey="Gordon, Richard" sort="Gordon, Richard" uniqKey="Gordon R" first="Richard" last="Gordon">Richard Gordon</name><name sortKey="Tunny, Terry" sort="Tunny, Terry" uniqKey="Tunny T" first="Terry" last="Tunny">Terry Tunny</name></country><country name="France"><region name="Île-de-France"><name sortKey="Pinet, Florence" sort="Pinet, Florence" uniqKey="Pinet F" first="Florence" last="Pinet">Florence Pinet</name></region><name sortKey="Corvol, Pierre" sort="Corvol, Pierre" uniqKey="Corvol P" first="Pierre" last="Corvol">Pierre Corvol</name><name sortKey="Rioual Aroff, Nathalie" sort="Rioual Aroff, Nathalie" uniqKey="Rioual Aroff N" first="Nathalie" last="Rioual-Caroff">Nathalie Rioual-Caroff</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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